CreatinineClearance:Enzymaticvs Jaff#{233} Determinationsof Creatininein Plasmaand Urine
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چکیده
staff Coomassie Brilliant Blau G250. J Clin Chem Cm Biochem 1981;19:203-8. 5. Peterson GL Determination of total protein.Methods Enzymol 1983;91:95-119. 6. SedmakJJ,GroesbergSE.A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250. Anal Biochem 1977;79:544-52. 7. JohnsonA, Lott JA. Standardization of the Coomassie Blue method forcerebrospinal fluid proteins. Clin Chem 197824:1931-3. 8. Pierce J, Juelter CH. An evaluationof the CoomassieBrilliant Blue G-250dyebinding method for quantitative protein determination. Anal Biochem 1977;81:478-80. 9. van Kley H, Hale SM. Assay for protein by dye binding. Anal Biocheni 1977;81:485-7. 10. Macart M, Gerbaut L. Improved dye-binding procedure-once again, or oncetoo much?[Letter]. Cliii Chem 1985;31:651-2. 11. Manuel Y, Revillard JP, Betuel H. Proteins in normal and pathological urine. Baltimore: University Park Press, 1970;355. 12. ShibaKS, Kanamori K, Harada T, et al. A causeofdiscrepancy
منابع مشابه
CombinedEnzymic - Jaff # { 233 } Methodfor Determinationof Creatininein Serum
Concentrations of creatinine, as determined in serum by a method involving the combined use of creatinine amidohydrolase (EC 3.5.2.10) and alkaline sodium picrate were found to be factitiously low, owing to a reversal of the enzyme reaction. This effect could be eliminated by converting creatine, the product of the enzymic reaction, to creatine phosphate. The combined enzymic-Jaff#{233} method ...
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The Jaff#{233} reaction is commonly used to determine the concentration of creatinine in urine and serum by measuring the color produced when picric acid and sodium hydroxide solutions are combined with urine or protein-free filtrate from serum. As is well known, substances other than creatinine react to give positive error, the relative amount of interference usually being much greater in seru...
متن کاملDevelopment and validation of a liquid-chromatographic procedure for serum creatinine.
We evaluated and compared three analytical procedures for measuring creatinine by testing pooled sera and sera from individual human subjects. In two of the methods, an enzymic and a conventional colorimetric procedure, creatinine was separated from serum proteins with tungstic acid and detected at 520 nm after reaction with alkaline picrate (Jaff#{233} reagent). The amount of enzymaticaIly act...
متن کاملAutomated reaction-rate method for determination of serum creatinine with the CentrifiChem.
An automated kinetic method to determine creatinine in serum with the Jaff#{233} reaction is described. The method does not require deproteinization or adsorption of creatinine on Fuller’s earth or ion.exchange resin. A maximum of 29 serum samples, standards, and control sera can be assayed in less than 1.5 mm. Interferences by most of the known Jaff#{233}-positive serum constituents were studi...
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A similar expression would result if different dilution steps were used for each ampoule or different coefficients of variation were used for different dilution steps or solutions. The sum above (S2) would include terms (1 d)2(Cv2 + CA2) with appropriate values of d, Cv and CA for each step. If R is the estimate of the relative potency computed for an assay, then for known relative potency, r, ...
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